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Intercellular adhesion molecule‐1 (ICAM‐1) expression is necessary for monocyte adhesion to the placental bed endothelium and is increased in type 1 diabetic human pregnancy
Author(s) -
Xie Lijuan,
Galettis Anoula,
Morris Jonathan,
Jackson Christopher,
Twigg Stephen M.,
Gallery Eileen D. M.
Publication year - 2007
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.793
Subject(s) - intercellular adhesion molecule 1 , cell adhesion molecule , monocyte , adhesion , icam 1 , cell adhesion , endothelium , microbiology and biotechnology , neural cell adhesion molecule , intercellular adhesion molecule , soluble cell adhesion molecules , biology , immunology , endocrinology , andrology , medicine , chemistry , organic chemistry
Background That adhesion molecule expression is upregulated in endothelial cells of the placental bed in pregnancies complicated by type 1 diabetes mellitus, and that this is associated with increased adherence of peripheral blood monocytes, which can be reversed by reduction in activity or expression of relevant adhesion molecules. Specific aims were to compare the adherence of monocytes from normal pregnancies to decidual endothelial cells from both normal and diabetic pregnancies, and to examine the involvement of intercellular adhesion molecule‐1 (ICAM‐1) and vascular cell adhesion molecule‐1 (VCAM‐1) in regulation of such adhesion. Methods We examined adhesion of peripheral blood monocytes (isolated by density gradient centrifugation) of normal third trimester pregnant women, to cultured endothelial cells (isolated from decidual biopsies collected at elective caesarean section) from both normal women and those with type 1 diabetes. Adhesion molecule expression was determined by flow cytometry. The role of ICAM‐1 was further investigated by monoclonal antibody‐blocking experiments and gene‐silencing methodology. Results There was a significant increase in monocyte adhesion to decidual endothelial cells from diabetic pregnancies, associated with increased endothelial cell expression of ICAM‐1, but not VCAM‐1. ICAM‐1 expression in normal decidual endothelial cells was stimulated by pro‐atherogenic and pro‐inflammatory stimuli. Following ICAM‐1 antibody blockade, monocyte adhesion was decreased by > 70%. ICAM‐1 silencing by small interfering RNAs also inhibited monocyte adhesion and ICAM‐1 expression. Conclusions These findings implicate upregulation of ICAM‐1 in decidual endothelial cells in the development of placental bed vascular pathology in diabetic pregnancy. Copyright © 2007 John Wiley & Sons, Ltd.