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Diabetes per se and metabolic state influence gene expression in tissue‐dependent manner of BB/OK rats
Author(s) -
Klöting Nora,
Follak Niels,
Klöting Ingrid
Publication year - 2004
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.526
Subject(s) - endocrinology , medicine , diabetes mellitus , insulin , spleen , gene expression , downregulation and upregulation , biology , gene , biochemistry
Abstract Background Several epidemiologic studies have clearly established that long‐term near normoglycaemia strongly protects against onset and progression of late complication of diabetes. Therefore, insulin treatment plays a crucial role in determining the quality of life of affected individuals. Here we studied the effects of exogenous insulin on gene expression levels in well‐ and poorly compensated diabetic subjects in comparison to non‐diabetic BB/OK rats to find out whether diabetes per se and the quality of insulin treatment have an effect on gene expression and whether it is tissue specific. Methods Six non‐diabetic and 12 diabetic BB/OK rats were studied. Diabetic subjects were either treated with insulin implants (well compensated) or treated with 1U insulin daily (poorly compensated) to guarantee survival. Four weeks after onset of diabetes, the animals were killed and expression of Yy1, Pparγ, Nfκb, Pref‐1, Tgfb1, Il‐10 , and Lepr was measured in thymus, spleen, liver, heart, and bone. Results In general, between diabetic and non‐diabetic subjects, significant expression changes were detected in spleen for Il‐10 , in heart for Il‐10 and Pparγ , in liver for Yy1, Nfκb , and Lepr , as well as in bone for all genes studied except Tgfb1. Except Lepr , no expression changes were observed in thymus. Between well‐ and poorly compensated rats, significant differences on expression level were found for Yy1 (liver), Pparγ (heart), Nfκb (bone) , Pref‐1 (spleen), and Lepr (thymus, liver, heart). Conclusion The insulin treatment compensates not only metabolic disturbances but also changes gene expression profile in BB/OK rats in a tissue‐dependent manner. Copyright © 2004 John Wiley & Sons, Ltd.