Premium
The changes in miR‐130b levels in human serum and the correlation with the severity of diabetic nephropathy
Author(s) -
Lv Chuan,
Zhou Yuehong,
Wu Can,
Shao Ying,
Lu Canlu,
Wang Qiuyue
Publication year - 2015
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.2659
Subject(s) - medicine , diabetic nephropathy , creatinine , microalbuminuria , endocrinology , type 2 diabetes mellitus , diabetes mellitus , blood urea nitrogen , triglyceride , insulin resistance , renal function , cholesterol
Background Circulating microRNA 130b has been closely associated with multiple diseases in humans such as cancer, obesity and diabetes mellitus. This study evaluates the correlation between serum miR‐130b and the severity of diabetic nephropathy evaluated by measurement of albuminuria. Methods Three hundred twenty‐seven patients with type 2 diabetes mellitus (T2DM) were divided into three groups: normoalbuminuria group [diabetes mellitus, urinary albumin to creatinine ratio (UACR) < 30 mg/g, n = 137], microalbuminuria group (DN1, UACR 30–300 mg/g, n = 122) and macroalbuminuria group (DN2, UACR > 300 mg/g, n = 68). The levels of serum miR‐130b were validated by real‐time polymerase chain reaction. Serum transforming growth factor β 1 (TGF‐ β 1), hypoxia inducible factor 1 α (HIF‐1 α ) and fibronectin were determined by enzyme‐linked immunosorbent assay. Results Compared with control, serum miR‐130b levels were significantly decreased in T2DM patients and further decreased in the patients of diabetes mellitus, DN1 and DN2 groups ( p < 0.001). Furthermore, age‐adjusted and sex‐adjusted regression analyses showed that decreased level of serum miR‐130b, increased levels of glycated haemoglobin (HbA 1c ), homeostatic model assessment of insulin resistance (HOMA‐IR), triglyceride (TG), low‐density lipoprotein (LDL), serum creatinine, blood urea nitrogen (BUN), TGF‐ β 1, HIF‐1 α and fibronectin were significantly correlated with UACR ( p < 0.05). In addition, serum miR‐130b levels were inversely correlated with HbA 1c , HOMA‐IR, TG, LDL, BUN, TGF‐ β 1, HIF‐1 α and FN ( p < 0.05). Conclusion Our findings suggest that serum miR‐130b may be a new biomarker for the early diagnosis of DN in T2DM. Circulating miR‐130b may possibly be involved in the pathological mechanism of DN, such as lipid metabolic disorders, oxidative stress, extracellular matrix deposition and renal fibrosis. Copyright © 2015 John Wiley & Sons, Ltd.