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Urinary mRNA levels of ELR‐negative CXC chemokine ligand and extracellular matrix in diabetic nephropathy
Author(s) -
Wang Gang,
Lai Fernand MacMoune,
Chow KaiMing,
Kwan Bonnie ChingHa,
Pang WingFai,
Luk Cathy ChoiWan,
Leung ChiBon,
Li Philip KamTao,
Szeto CheukChun
Publication year - 2015
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.2654
Subject(s) - diabetic nephropathy , chemistry , nephropathy , ligand (biochemistry) , extracellular matrix , urinary system , chemokine , medicine , diabetes mellitus , endocrinology , biochemistry , receptor
Abstract Background Inflammation and fibrosis play important roles in the progression of diabetic nephropathy. We determine the urinary mRNA levels of ELR‐CXC chemokine ligand and extracellular matrix in diabetic nephropathy. Methods We studied 26 patients with biopsy‐proven diabetic nephropathy, 15 with hypertensive nephrosclerosis and 10 healthy controls. Urinary mRNA levels of CXCL9, CXCL10, CXCL11, collagen I A1 chain, collagen IV A3 chain and fibronectin were measured. Patients were followed for 36.9 ± 7.4 months to determine the rate of glomerular filtration rate (GFR) decline. Results Urinary mRNA levels of CXCL10 and CXCL11 are decreased, and those of collagen I A1 chain and fibronectin are increased in diabetic nephropathy. Baseline estimated GFR correlates with urinary mRNA level of CXCL9 ( r = 0.583, p = 0.002) and CXCL11 ( r = 0.703, p < 0.0001), respectively. The rate of GFR decline significantly correlates with urinary CXCL9 ( r = −0.618, p = 0.0008) and CXCL11 mRNA levels ( r = −0.726, p < 0.0001). Multivariate linear regression analysis confirms that urinary CXCL9 mRNA level is independently associated with the rate of GFR decline, while the correlation with urinary CXCL11 mRNA level has borderline significance. Conclusion Urinary CXCL9 and CXCL11 mRNA levels correlate with baseline renal function. The rate of renal function decline correlates with urinary CXCL9 mRNA level. Our results suggest that urinary CXCL9 mRNA levels may be used for risk stratification of diabetic nephropathy. Copyright © 2015 John Wiley & Sons, Ltd.