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β ‐Carotene, α ‐tocopherol and ascorbic acid: differential profile of antioxidant, inflammatory status and regulation of gene expression in human mononuclear cells of diabetic donors
Author(s) -
Oliveira Barbara Fonseca,
Costa Daniela Caldeira,
NogueiraMachado José Augusto,
Chaves Miriam Martins
Publication year - 2013
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.2439
Subject(s) - antioxidant , ascorbic acid , oxidative stress , superoxide dismutase , reactive oxygen species , chemistry , nadph oxidase , nicotinamide adenine dinucleotide phosphate , proinflammatory cytokine , vitamin c , biochemistry , oxidase test , inflammation , biology , enzyme , immunology , food science
Background Diabetic patients are exposed to increased oxidative stress due to several mechanisms, mainly hyperglycaemia. Pathological processes, such as those in type 1 diabetes, include diminished activity of the antioxidant defense system(s) or excessive oxidative generation resulting in an oxidative/antioxidant imbalance and development of oxidative stress. Methods The purpose of this study was to evaluate the production of reactive oxygen species (ROS) (chemiluminescence) and reduction capacity (MTT dye reduction), the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits, superoxide dismutase and catalase using quantitative reverse‐transcriptase polymerase chain reaction, and the levels of cytokines [interleukin (IL)‐6, tumour necrosis factor‐ α , IL‐8, IL‐10 and IL‐4] by sandwich enzyme‐linked immunosorbent assay in mononuclear cells from non‐diabetic and diabetic donors treated with a vitamin complex (ascorbic acid, β ‐carotene and α ‐tocopherol) in two different concentrations ([A] = ascorbic acid = 0.08 µM, α‐tocopherol = 0.04 µM, β‐carotene = 0.0008 µM and [20A] = ascorbic acid = 1.6 µM, α‐tocopherol = 0.82 µM, β‐carotene = 0.016 µM). Results Concentration [A] was antioxidant reducing ROS production, expression of NADPH oxidase subunits and pro‐inflammatory cytokines while raising the expression of antioxidant enzymes and reducing pro‐inflammatory cytokines in both groups. Concentration [20A] was pro‐oxidant by raising ROS production, NADPH oxidase subunits and pro‐inflammatory cytokines and reducing antioxidant enzymes and anti‐inflammatory cytokines in the non‐diabetic group but antioxidant in cells of type 1 diabetic patients by raising antioxidant enzymes and anti‐inflammatory cytokines and reducing pro‐inflammatory cytokines. Conclusion The vitamin complex has a dual effect, pro‐oxidant and antioxidant, being also dose dependent with different profiles of cells of non‐diabetic and type 1 diabetic patients. Copyright © 2013 John Wiley & Sons, Ltd.