Premium
Nonenzymatic glycation of high‐density lipoprotein impairs its anti‐inflammatory effects in innate immunity
Author(s) -
Liu Donghui,
Ji Liang,
Zhang Dongmei,
Tong Xunliang,
Pan Bing,
Liu Pinli,
Zhang Youyi,
Huang Yining,
Su Jinzi,
Willard Belinda,
Zheng Lemin
Publication year - 2012
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.1297
Subject(s) - glycation , endocrinology , medicine , chemistry , tumor necrosis factor alpha , lipopolysaccharide , lipoprotein , high density lipoprotein , rage (emotion) , diabetes mellitus , innate immune system , inflammation , cholesterol , biology , receptor , neuroscience
Aims/hypothesis In type 2 diabetes mellitus (T2DM), the abnormal protein and lipid composition of diabetic high‐density lipoprotein (HDL) could impair its anti‐inflammatory functions. Whether nonenzymatic glycation directly impaired the anti‐inflammatory effects of HDL in innate immunity remained unclear. Methods Human acute monocytic leukemia cell line (THP‐1) cells, mouse RAW 264.7 macrophages and primary human monocytes derived macrophages were pre‐incubated with native HDL, diabetic HDL isolated from T2DM patients or HDL glycated with different doses of d ‐glucose in vitro and then challenged with lipopolysaccharide (LPS). The release of tumor necrosis factor (TNF)‐ α and IL‐1 β was assayed by enzyme‐linked immunosorbent assay (ELISA). Phosphorylation of I κ ‐B α in cytoplasm and nuclear translocation of NF‐ κ B were detected by western blot. Glycation levels of native HDL, glycated HDL and diabetic HDL were determined using LC‐MS/MS. Results The potency of diabetic HDL to inhibit the release of TNF‐ α ( p < 0.05) and IL‐1 β ( p < 0.001) was dramatically attenuated compared with that of native HDL. Similarly, glycation of HDL in vitro impaired its ability to inhibit TNF‐ α and IL‐1 β release in a glucose dose‐dependent manner. Moreover, apoHDL still effectively inhibited the release of TNF‐ α and IL‐1 β induced by LPS, but glycated apoHDL partly lost such abilities. Nonenzymatic glycation levels of glycated HDL and diabetic HDL increased 28 fold ( p < 0.001) and 4 fold ( p < 0.001), respectively compared with that of native HDL. Conclusions In this study, we observed that diabetic HDL and HDL glycated in vitro both partly lose their protective effects to inhibit cytokines release induced by LPS in macrophages, and nonenzymatic glycation of the protein components of HDL plays key roles in these impairments. Copyright © 2011 John Wiley & Sons, Ltd.