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The development of Na + ‐dependent binding of γ‐aminobutyric acid to particles of rat brain
Author(s) -
Defeudis F. V.
Publication year - 1973
Publication title -
developmental psychobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 93
eISSN - 1098-2302
pISSN - 0012-1630
DOI - 10.1002/dev.420060312
Subject(s) - aminobutyric acid , chemistry , homogenization (climate) , medicine , fractionation , endocrinology , biophysics , biochemistry , biology , chromatography , receptor , biodiversity , ecology
The Na + ‐dependent binding of γ‐aminobutyric acid (GABA) in rat cerebral hemispheres was studied by subcellular fractionation and double‐labelling procedures. The distributions of tracer amounts of [2,3‐ 3 H] GABA and [U‐ 14 C] surcrose acquired by P 1 (crude nuclear) and P 2 (synaptosomal, mitochondrial) particles during homogenization at O° C were compared in the presence of 40 m M Na + and in the absence of added Na + . Results indicated that addition of Na + to the homogenizing fluid promotes extra binding of GABA to both P 1 and P 2 particles and that these processes are age‐dependent, but different. GABA binding to P 1 particles increased with age until 21‐22 days after which it remained rather constant. On the other hand, a more dramatic developmental change occurred in the binding of GABA to P 2 fractions (which contain the majority of the nerve endings) which was maximal in the brains of rats 11 and 15 days old and which declined in older rats.