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Deletion of c16orf45 in zebrafish results in a low fertilization rate and increased thigmotaxis
Author(s) -
Li Tingting,
Li Fei,
Lin Jia,
Zhang Yinglan,
Zhang Qi,
Sun Yanhe,
Chen Xudong,
Xu Mingqing,
Wang Xu,
Li Qiang
Publication year - 2020
Publication title -
developmental psychobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 93
eISSN - 1098-2302
pISSN - 0012-1630
DOI - 10.1002/dev.21984
Subject(s) - zebrafish , human fertilization , thigmotaxis , biology , anatomy , genetics , endocrinology , gene , open field
Abstract c16orf45 is located at 16p13.11, an important locus related to neurodevelopmental diseases. Clinical studies have demonstrated that c16orf45 is associated with various neurodevelopmental diseases. To further elucidate the effect of c16orf45 on neural development, we constructed a zebrafish model with a stably inherited c16orf45 deletion via CRISPR/Cas9 technology. We found that deletion of c16orf45 significantly reduced the zebrafish fertilization rate, and both females and males showed reduced fertility. Meanwhile, the homozygous c16orf45 knockout zebrafish showed a developmental delay at 24 hr postfertilization (hpf). However, morphological changes were not apparent after 2 days postfertilization (dpf). Notably, the results of behavioral experiments revealed increased thigmotaxis in c16orf45 − / − zebrafish at 2 months. In conclusion, these findings demonstrate that c16orf45 plays an important role in nervous system and reproductive system.