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Application of one‐bead one‐structure approach to identification of nonpeptidic ligands
Author(s) -
Staňková Magda,
Issakova Olga,
Sepetov Nikolai F.,
Krchňák Viktor,
Lam Kit S.,
Lebl Michal
Publication year - 1994
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.430330211
Subject(s) - moiety , chemistry , solid phase synthesis , streptavidin , alkylation , residue (chemistry) , imidazole , combinatorial chemistry , stereochemistry , chemical synthesis , amino acid , organic chemistry , biochemistry , peptide , in vitro , biotin , catalysis
A synthetic chemical library comprised of alkylated and acylated amino acids was synthesized and screened to determine structures that bind to a model target, streptavidin. The library was prepared using “split synthesis” and screened in a solid phase binding assay. The structure of positively reacting compounds was determined using mass spectroscopy. Positive compounds, together with various structural analogs were synthesized and their binding confirmed. Structures containing both an imidazole moiety and a substituted aromatic residue demonstrated binding. © 1994 Wiley‐Less, Inc.