Premium
Effect of glycosides of Streblus asper on motility, glucose uptake, and certain enzymes of carbohydrate metabolism of Setaria cervi
Author(s) -
Singh Som N.,
Chatterjee Ranjit K.,
Srivastava Arvind K.
Publication year - 1994
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.430320309
Subject(s) - glycoside , biology , glucokinase , carbohydrate metabolism , biochemistry , metabolism , enzyme , dehydrogenase , carbohydrate , in vitro , botany
The in vitro effects of two pure glycosides, i.e., asperoside (K029) and strebloside (K030) isolated from a traditionally used medicinal plant Streblus asper on the bovine filarial parasite Setaria cervi females were studied. Both K029 and K030 caused death of the worms within 2 to 3 hours at concentrations of 10 μg/ml (1.7 pmoles) and were found to inhibit motility and glucose uptake of the parasites at lower concentrations (0.1 μg/ml; 0.17 pmoles). These glycosides also inhibited the incorporation of [U‐ 14 ]C‐glucose into macromolecules of S. cervi females. Parasites preincubated with either K029 or K030 had lowered profiles of glucokinase (EC 2.7.1.2), malate dehydrogenase (EC 1.1.1.37) and succinate dehydrogenase (EC 1.3.99.1) activities, suggesting that the lethal effects of the glycosides were due to effects on glucose metabolism.