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Effects of chlorisondamine on nicotinic receptor binding in whole brain and nicotine‐induced changes in locomotor activity in rats
Author(s) -
Decker Michael W.,
Majchrzak Mark J.,
Cadman Evelyn D.,
Arnerić Stephen P.
Publication year - 1994
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.430310202
Subject(s) - chlorisondamine , nicotinic agonist , nicotine , chemistry , endocrinology , cholinergic , receptor , pharmacology , medicine , acetylcholine receptor , antagonist , microviscosity , anabasine , biochemistry , biology , blood pressure , membrane
Chlorisondamine, a nicotinic cholinergic receptor antagonist, can block many of the in vivo effects of nicotine for weeks after a single icv injection. The time course of this effect was examined in a single group of rats by assessing the effects of nicotine on locomotor activity at 1, 3, and 6 weeks after the icv administration of 23 nmol of chlorisondamine. The effects of nicotine on locomotor activity were biphasic as has been previously reported, with decreases in activity early in the session and increases in activity later in the session. These effects of nicotine were blocked in chlorisondamine‐treated rats at 1 or 3 weeks but not 6 weeks after administration of chlorisondamine. Nicotinic receptor binding in the brains of chlorisondamine‐treated rats revealed no change in B max but a significant increase in affinity (47–59% decrease in K d ) 1, 3, 6, or 7 weeks after treatment. In contrast to its effects on affinity when administered icv, chlorisondamine did not alter binding affinity when added directly to the incubation buffer in vitro. Thus, although chlorisondamine significantly alters neuronal nicotinic cholinergic receptor binding affinity, this effect of chlorisondamine on binding affinity does not appear to be a direct effect of chlorisondamine on the receptor or to match the time course of chlorisondamine blockade of nicotine‐induced changes in locomotor activity. © 1994 Wiley‐Liss, Inc.