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Adenosine outflow from hippocampal slices evoked by ischemic‐like conditions: Effect of the excitatory amino acid antagonists
Author(s) -
Pedata Felicita,
Latini Serena,
Pazzagli Marta,
Pepeu Giancarlo
Publication year - 1993
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.430280338
Subject(s) - adenosine , inosine , nmda receptor , chemistry , dnqx , glutamate receptor , adenosine a1 receptor , adenosine receptor , pharmacology , excitatory postsynaptic potential , medicine , endocrinology , biochemistry , receptor , biology , ampa receptor , agonist
The effect of amino acid receptor antagonists on adenosine and inosine outflow evoked by 5 or 10 min ischemia‐like conditions “in vitro” (glucose‐free Krebs solution gassed with 95% N 2 /5% CO 2 ) was investigated on rat hippocampal slices. The viability of the slices after ischemia was evaluated by extracellular recording of the evoked synaptic responses in the CA1 region. The NMDA receptor antagonist D(−)‐2‐amino‐7‐phosphonoheptanoic acid (D‐AP7 100 μM) and the non‐NMDA receptor antagonist 6,7‐dinitroquinoxaline‐2,3‐dione (DNQX 10 μM) did not significantly decrease the adenosine and inosine outflow evoked by ischemia. The glutamate uptake blocker dihydrokainate (500 μM) also did not affect the outflow of adenosine and inosine. It is concluded that ischemia‐like conditions “In vitro” evoke and adenosine and inosine outflow, through a mechanism which does not involve excitatory amino acids. © 1993 Wiley‐Liss, Inc.