Premium
Diurnal variation in hepatic glutathione content as a function of age
Author(s) -
Rikans Lora E.,
Nokubo Munetaka,
Kanai Setsuko,
Kitani Kenichi
Publication year - 1992
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.430260409
Subject(s) - glutathione , medicine , endocrinology , circadian rhythm , diurnal temperature variation , chemistry , hepatic function , biology , physiology , biochemistry , enzyme , atmospheric sciences , geology
The purpose of this study was to investigate the influence of aging on the diurnal varitaion in glutathione (GSH) content of mouse liver. Young (7 months) and old (27 months) C57BL mice were housed under standarized conditions with lighting from 0800 to 2000 and free access to food and water. GSH was determined in acid extracts of liver homogenates by HPLC separation and fluorometric detection of the o‐phthaldialdehyde derivative. A diurnal rhythm in hepatic GSH concentration was evident in young and old mice of both sexes. Peak levels of GSH occurred at 1000 and were unaffected by age or gender; however, age and sex‐dependent differences were observed in the lowest concentration of GSH and in the amplitude of the GSH cycle. Hepatic GSH levels at 2200 and 0600 were 30–35% lower in old males than in young males and 25–40% lower in young females than in young males. The results may provide an explanation for the inconsistencies in previous findings about the influence of aging on hepatic GSH concentrations. © 1992 Wiley‐Liss, Inc.