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In vivo hemodynamic effects of endothelium‐derived constricting factor in the dog
Author(s) -
Hom Gary J.,
Highsmith Robert F.,
Pang David C.
Publication year - 1989
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.430180205
Subject(s) - coronary perfusion pressure , aortic pressure , cardiology , medicine , perfusion , ventricular pressure , vasoconstriction , hemodynamics , coronary arteries , blood pressure , endothelium , coronary circulation , anesthesia , artery , blood flow , cardiopulmonary resuscitation , resuscitation
Primary cultures of bovine thoracic aortic endothelial cells release an endothelium‐derived constricting factor (EDCF), which contracts isolated porcine, bovine, and canine coronary arteries and increases coronary perfusion pressure in isolated rabbit heart. We have tested the effect of theis EDCF on coronary and aortic flows, left ventricular dP/dt, and left ventricular end diastolic pressure, and mean arterial pressure in opern chest anesthetized dogs. Intracoronary administration of conditioned medium containing EDCF caused a reduction in coronary flow. The constrictor response was slow in onset, peaked 50 min after bolus injection, and was sustained during the rest of the observation period. Aortic flow, heart rate, left ventricular dP/dt, left ventricular end diastolic pressure, and mean arterial pressure were not significantly altered. Intracoronary administration of denatured conditioned medium or nonconditioned medium did not elicit any cardiovascular changes. The EDCF‐induced reduction in coronary flow was inhibited by intracoronary administration of nitroglycerin. These findings suggest that conditioned medium from bovine aortic endothelial cells produces coronary vasoconstriction in the intact, anesthetized dog.