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Introduction to RNA ‐Seq and its Applications to Drug Discovery and Development
Author(s) -
Khatoon Zainab,
Figler Bryan,
Zhang Hui,
Cheng Feng
Publication year - 2014
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.21215
Subject(s) - transcriptome , computational biology , drug discovery , rna seq , gene , biology , rna splicing , rna , alternative splicing , microrna , drug development , exon , non coding rna , gene expression , genetics , bioinformatics , drug , pharmacology
Preclinical ResearchRNA sequencing ( RNA ‐ Seq ) is a novel high‐throughput technology for comprehensive transcriptome study. It can measure the expression levels of thousands of genes simultaneously and provide insight into functional pathways and regulations in biological processes. In addition, RNA ‐Seq can provide copious information on alternative splicing, allele‐specific expression, unannotated exons, and novel transcripts (gene or noncoding RNAs ). This technology has revolutionized the way biologists examine transcriptomes and has been successfully applied in drug discovery and development, being able to identify drug‐related genes, microRNAs , and fusion proteins. In this overview, we will review this technology including data analysis, and its recent applications in drug discovery and development.