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Anti‐Inflammatory and Antiproliferative Activities of Organic Fractions from the Mediterranean Brown Seaweed, C ystoseira Compressa
Author(s) -
Mhadhebi Lamia,
Dellai Afef,
ClaryLaroche Audrey,
Said Rafik Ben,
Robert Jacques,
Bouraoui Abderrahman
Publication year - 2012
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.20491
Subject(s) - pharmacology , carrageenan , chemistry , ethyl acetate , in vivo , mtt assay , in vitro , biochemistry , medicine , biology , microbiology and biotechnology
trategy, Management and Health PolicyEnabling Technology, Genomics, Proteomics Preclinical Research Preclinical Development Toxicology, Formulation Drug Delivery, Pharmacokinetics Clinical Development Phases I‐III Regulatory, Quality, Manufacturing Postmarketing Phase IVThe inability to cure many diseases, such as cancer and arthritis, has stimulated the need for the development of new drugs from natural sources. Of all natural sources, the marine environment is clearly the last great frontier for pharmaceutical and medical research. As part of our search for new anti‐inflammatory or anticancer potential drugs, organic fractions (chloroform, ethyl acetate and methanol) from the M editerranean brown seaweed, C ystoseira compressa were evaluated for in vivo anti‐inflammatory activity, using the carrageenan‐induced rat paw edema model and in vitro antiproliferative effect in three human cancer cell lines ( A 549, lung cell carcinoma; HCT 15, colon cell carcinoma; and MCF 7, breast adenocarcinoma), using an MTT assay. The chloroform and ethyl acetate fractions exhibited dose‐dependent anti‐inflammatory activity, comparable with the reference drug acetylsalicylic–lysine (300 mg/kg; i.p.). The percent inhibition of edema, 3 h after carrageenan injection ranged from 63 to 76% and 65 to 78%, respectively. The organic fractions ( F‐CHCl 3, F‐EtOAC , and F‐MeOH ) also exhibited antiproliferative activity against the three human cancer cell lines with IC 50 values ranging from 78–82 μg/ml; 27–50 μg/ml and 110–130 μg/ml; respectively. Thus, the chloroform and ethyl acetate fractions of C . compressa evidenced an interesting anti‐inflammatory activity associated with significant antiproliferative activity, efficacies that correlated with their total phenol content. The purification and the determination of chemical structures of compounds of these active fractions are under investigation.