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Diclofenac acid: a free‐radical‐scavenger to protect DNA against radical‐induced oxidation
Author(s) -
Tang YouZhi,
Liu ZaiQun
Publication year - 2009
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.20324
Subject(s) - chemistry , radical , abts , tbars , antioxidant , diclofenac sodium , scavenger , dna oxidation , hydroxyl radical , thiobarbituric acid , dpph , lipid peroxidation , medicinal chemistry , nuclear chemistry , organic chemistry , chromatography , oxidative damage
The present work focused on the antioxidant effects of diclofenac acid (DaH) and its sodium salt (DaNaH) on the radical‐induced oxidation of DNA. 2,2′‐Azobis(2‐amidinopropane) dihydrochloride (AAPH) was used as radical initiator to oxidize naked DNA sodium salt, followed by the determination of thiobarbituric acid reactive substance (TBARS). DaH and DaNaH also interacted with two other radicals: 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonate) radical cation (ABTS +. ) and 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH). DaH and DaNaH produce a concentration‐dependent protection of DNA. Kinetic studies established that either DaH or DaNaH trap 3–4 radicals when they protect DNA against AAPH‐induced oxidation. DaH and DaNaH scavenged ABTS +. efficiently. Diclofenac was thus found to be an antioxidant that concentration‐dependently reduced radicals rather than donated its hydrogen atom to radicals. Drug Dev Res 70: 520–524, 2009. © 2009 Wiley‐Liss, Inc.