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Estradiol‐induced enhancement in cell proliferation is mediated through estrogen receptors in the dentate gyrus of adult female rats
Author(s) -
Nagy Anna I.,
Ormerod Brandi K.,
Mazzucco Christine,
Galea Liisa A.M.
Publication year - 2005
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.20053
Subject(s) - dentate gyrus , cell growth , medicine , endocrinology , ovariectomized rat , estrogen receptor , estrogen , estradiol benzoate , receptor , chemistry , biology , hippocampus , biochemistry , cancer , breast cancer
High‐level estradiol enhances cell proliferation in the dentate gyrus of adult female rats within 4 h of administration and then suppresses cell proliferation within 48 h via an adrenal steroid‐dependent mechanism (Ormerod et al., [2003b] J Neurobiol 55:247–260). Here, we investigate whether the estradiol‐induced enhancement in progenitor cell proliferation is mediated through estrogen receptors (ERSs) using the selective ER antagonist ICI 182,780 (ICI). Ovariectomized Sprague‐Dawley rats were given two subcutaneous injections of either vehicle + vehicle (VEH; 0.1 ml sesame oil); VEH+ICI (500 µg); estradiol benzoate (EB; 10 µg)+VEH; or EB +ICI. The cell synthesis marker, 5‐bromo‐2′‐deoxyuridine (200 mg/kg) was administered 4 h later. Animals were perfused 24 h after BrdU injection and cell proliferation was assessed following immunohistochemical processing of the tissue. Relative to VEH, EB increased cell proliferation by approximately 50%. This EB‐induced increase was partially blocked by ICI 182,780 treatment, and ICI 182,780 treatment alone enhanced cell proliferation. Our results demonstrate that estradiol enhances cell proliferation in the dentate gyrus of adult female rats by activating estrogen receptors. Drug Dev. Res. 66:142–149, 2006. © 2006 Wiley‐Liss, Inc.