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Modulation of the A 3 adenosine receptor by low agonist concentration induces antitumor and myelostimulatory effects
Author(s) -
Merimsky Ofer,
BarYehuda Sara,
Madi Lea,
Fishman Pnina
Publication year - 2003
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.10182
Subject(s) - protein kinase b , signal transduction , agonist , downregulation and upregulation , cancer research , pi3k/akt/mtor pathway , chemistry , endocrinology , biology , receptor , medicine , microbiology and biotechnology , biochemistry , gene
The A 3 adenosine receptor (A 3 AR) agonists IB‐MECA and Cl‐IB‐MECA act as anticancer agents by inhibiting the growth of melanoma and colon carcinoma cells both in vitro and in vivo. The A 3 AR is highly expressed in these tumor cells and upon agonist binding receptor internalization and recycling occurs. The downstream signaling pathways that follow these events include a decrease in the expression level of protein kinase A (PKA) and protein kinase B/Akt (PKB/Akt) leading to the deregulation of the Wnt and the NF‐κB signaling pathways. Interestingly, IB‐MECA and Cl‐IB‐MECA generate the opposite, beneficial effect on myeloid cells. These agonists induce the production of granulocyte colony‐stimulating factor (G‐CSF) by murine splenocytes. The molecular mechanisms underlying the events prior to G‐CSF production include the upregulation of NF‐κB and the upstream kinases phosphoinositide 3‐kinase (PI3K), PKB/Akt, and IKK. In addition, a myeloprotective effect is noted upon administration of IB‐MECA and Cl‐IB‐MECA to chemotherapy‐treated mice. This was expressed by accelerated recovery of white blood cells and neutrophil counts in cyclophosphamide‐treated mice following agonist administration. Taken together, activation of the A 3 AR on tumor and normal cells generates opposing responses: in tumor cells, NF‐κB expression level decreases, resulting in tumor growth inhibition, while in normal cells it is upregulated, leading to G‐CSF production and the induction of myelostimulation. It thus appears that A 3 AR agonists act as tumor growth inhibitors, simultaneously maintaining the myeloid system, capable of preventing the damage of chemotherapeutic agents. Drug Dev. Res. 58:386–389, 2003. © 2003 Wiley‐Liss, Inc.