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Effect of lignans isolated from Hernandia nymphaeifolia on reactive oxygen species generation and calcium mobilization in human neutrophils
Author(s) -
Chao YuYing,
Jan ChungRen,
Ko YingChin,
Chen JihJung,
Chen IhSheng
Publication year - 2002
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/ddr.10042
Subject(s) - arachidonic acid , chemistry , reactive oxygen species , calcium , intracellular , biochemistry , phorbol , calcium in biology , protein kinase c , signal transduction , enzyme , organic chemistry
The effect of five lignans isolated from Hernandia nymphaeifolia on the reactive oxygen species (ROS) generation (O 2 – and H 2 O 2 ) and Ca 2+ mobilization in human neutrophils was investigated. The five lignans were epi‐yangambin, epi‐magnolin, epi‐aschantin, deoxypodophyllotoxin, and yatein. All five lignans (25–200 μM) suppressed the ROS generation induced by 20 μM N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) and 1 μM phorbol myristate acetate (PMA) without altering the ROS generation induced by 0.1 μM arachidonic acid. In Ca 2+ ‐containing medium, the lignans (50–100 μM) inhibited 20 μM fMLP‐ and 0.1 μM arachidonic acid‐induced increases in the intracellular free Ca 2+ levels ([Ca 2+ ] i ). In Ca 2+ ‐free medium, the lignans (50–100 μM) inhibited 0.1 μM arachidonic acid‐induced [Ca 2+ ] i increase without changing 20 μM fMLP‐induced [Ca 2+ ] i increase. In Ca 2+ ‐free medium, after depleting intracellular Ca 2+ stores with fMLP and arachidonic acid, addition of 3 mM Ca 2+ induced Ca 2+ influx. However, the lignans inhibited fMLP‐ and arachidonic acid‐induced Ca 2+ influx. Collectively, this study shows that the lignans alter ROS generation and Ca 2+ signaling in human neutrophils in a multiple manner. Drug Dev. Res. 55:118–126, 2002. © 2002 Wiley‐Liss, Inc.