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Cytologic detection of herpes simplex virus DNA in nipple discharge by in situ hybridization: Report of two cases
Author(s) -
Kobayashi Tadao K.,
Okamoto Hajime,
Yakushiji Michiaki
Publication year - 1993
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.2840090310
Subject(s) - in situ hybridization , herpes simplex virus , cytology , papanicolaou stain , hybridization probe , pathology , hsl and hsv , multinucleate , virus , in situ , giant cell , dna–dna hybridization , dna , staining , medicine , biology , virology , chemistry , cancer , genetics , gene expression , cervical cancer , organic chemistry , gene
Cytologic changes in the smears from nipple discharge of two cases with herpes simplex virus (HSV) infections are described. The cytology revealed the ground‐glass appearance of the nuclei with multinucleated syncytial cells. Subsequently, in situ hybridization (ISH) using a biotinytated DNA probe was applied to identify the HSV DNA in the Papanicolaou‐destained specimens. Positive hybridization was found with intense staining for the HSV DNA in the nuclei of cells having a ground‐glass appearance. Cytologic observation together with ISH procedure may be rapid and valuable tool for the detection and final demonstration of HSV infections. Similar investigations may be carried out in cellular samples from other body sites.