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Cytologic diagnosis of malignant mesothelioma in serous effusions using an antimesothelial‐cell antibody
Author(s) -
Donna Adalberto,
Betta PierGiacomo,
Bellingeri Domenico,
Tallarida Francesca,
Pavesi Mansueto,
Pastormerlo Massimo
Publication year - 1992
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.2840080411
Subject(s) - mesothelioma , serous fluid , medicine , pathology , immunostaining , cytology , antibody , immunohistochemistry , immunology
Differentiating mesothelioma, reactive mesothelium, and adenocarcinoma in serous effusions is often difficult, despite the application of ancillary techniques in support of the traditional cytomorphologic criteria. A polyclonal antimesothelial‐cell antibody recently developed by our group was evaluated as a histogenetic marker on a series of primary (n = 12) and metastatic (n=12) malignant effusions. Immunostaining was performed on paraffin sections from cell blocks. All mesothelioma effusions stained positive for the antibody, whereas, in contrast, all metastatic carcinoma specimens failed to react. These results (100 percent specificity and 100% sensitivity for mesothelioma) provide a basis for a reliable use of the antibody in the cytologic examination of suspicious or malignant serous effusions.