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Detection of Chlamydia Trachomatis in papanicolaou‐stained cervical smears: Control study by in situ hybridization
Author(s) -
Ghirardini Carla,
Ghinosi Patrizia,
Raisi Orville,
Rivasi Francesco,
Trentini Gian Paoio
Publication year - 1991
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.2840070221
Subject(s) - chlamydia trachomatis , papanicolaou stain , medicine , pap test , papanicolaou test , in situ hybridization , chlamydiales , chlamydia , chlamydiaceae , chlamydial infection , in situ , cytology , pap smears , carcinoma in situ , pathology , gynecology , cervical cancer , biology , carcinoma , cancer , immunology , cervical cancer screening , chemistry , biochemistry , gene expression , organic chemistry , gene
Ninety‐one Papanicolaou‐stained vaginopancervical smears were destained and subjected to in situ hybridization with Chlamydia trachomatis DNA probe. At cytologic examination (Pap test), 71 smears showed changes suggestive of chlamydial infection, while remaining 20 were negative. At the control by in situ hybridization, the results of Pap test were confirmed in 85 out of 91 cases, two false‐positive and four false‐negative cases being detected. The sensitivity and specifcity of Pap test, compared with in situ hybridization, were 95% and 89%, respectively. Like some recent reports, the present study confirms the reliability of Pap test in the detection of Chlamydia trachomatis and its possible relevant role in reducing the diffusion of the infection, when properly applied to mass‐screening program.