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Detection of human papillomavirus in reprocessed routine papanicolaou smears by dna hybridization
Author(s) -
Rakoczy P.,
Hutchinson L.,
Kulski J. K.,
Sterrett G. F.,
Pixley E. C.
Publication year - 1990
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.2840060313
Subject(s) - medicine , papanicolaou stain , human papillomavirus , papillomaviridae , gynecology , dna–dna hybridization , virology , dna , cervical cancer , genetics , cancer , biology
DNA dot blot hybridization was used for the detection of human papillomavirus (HPV) types 6, 11, 16, anti 18 in reprocessed routinely collected Papanicolaou smears. DNA was extracted from the smears with alkaline lysis and applied onto a nitrocellulose jilter. The specificity and sensitivity of the dot blot hybridization on reprocessed Papanicolaou smears were confirmed by Southern blot analysis of selected samples, using CaSki, SiHa cell lines as positive controls and HF 32 as negative controls. From 42 normal smears and 44 abnormal smears with koilocytosis present, 9 (21W) and 43 (98 %), respectively, were positive for HPV6/11/16 or 18. These results show that reprocessed Papanicolaou smears in combination with DNA hybridization have potential application in retrospective studies on the prevalence and distribution of HPV infection.