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Application of monoclonal antibody 44‐3A6 in the cytodiagnosis and classification of pulmonary carcinomas
Author(s) -
Banner Barbara F.,
Gould Victor E.,
Radosevich James A.,
Ma Yixing,
Lee Inchul,
Rosen Steven T.
Publication year - 1985
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.2840010408
Subject(s) - immunostaining , pathology , medicine , monoclonal antibody , adenocarcinoma , immunohistochemistry , cytology , monoclonal , antibody , cancer , immunology
Thirty‐five pulmonary carcinomas were studied retrospectively with monoclonal antibody (MCA) 44‐3A6 raised against a human adenocarcinoma cell line. The antibody was applied to cytologic smears of bronchial brushings originally stained with the Papanicolau method, and to conventional tissue sections. Ten of 12 adenocarcinomas (ADC) immunostained strongly in sections and smears, as did five of seven large‐cell „undifferentiated” carcinomas (LCUC). Eight neuroendocrine carcinomas (NEC) and eight squamous‐cell carcinomas (SCC) were negative, except for rare weakly positive foci. We conclude that MCA 44‐3A6 can be effectively applied on cytologic smears, and that it could be valuable in the precise classification of pulmonary carcinomas. The immunoreactivity of the ADC and SCC was predictable. Positive immunostaining in some LCUC confirms that these constitute a heterogeneus tumor class that includes cases that are phenotypically ADC despite the absence of obvious glands. Occasional immunostaining in NEC suggests focal exocrine differentiation as previously noted by electron microscopy. Diagn Cytopathol 1985;1:300–7.