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Improved antigen detection in ethanol‐fixed cytologic specimens. A modified avidin‐biotin‐peroxidase complex (ABC) method
Author(s) -
Gupta Prabodh K.,
Myers Joseph D.,
Baylin Stephen B.,
Mulshine James L.,
Cuttitta Frank,
Gazdar Adi F.
Publication year - 1985
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.2840010209
Subject(s) - medicine , avidin , peroxidase , biotin , cytology , antigen , ethanol , immunology , pathology , biochemistry , enzyme , chemistry
Abstract A new amplification technique is described for the detection of small amounts of antigen in ethanol‐fixed cytologic specimens and formalin‐fixed tissues. By adding a third antibody layer to the already sensitive avidin‐biotin immunoperoxidase system. we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.