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CD15, CD30, and PAX5 evaluation in Hodgkin's lymphoma on fine‐needle aspiration cytology samples
Author(s) -
Cozzolino Immacolata,
Vitagliano Giulio,
Caputo Alessandro,
Montella Marco,
Franco Renato,
Ciancia Giuseppe,
Selleri Carmine,
Zeppa Pio
Publication year - 2020
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.24366
Subject(s) - cd15 , pax5 , cd30 , medicine , pathology , lymphoma , cytology , hodgkin lymphoma , antibody , b cell , biology , immunology , cd34 , genetics , stem cell
Abstract Background The phenotypical identification of diagnostic cells is crucial for the diagnosis of Hodgkin's lymphoma (HL) on fine‐needle aspiration cytology (FNAC). The aim of this study is to evaluate the immunocytochemical (ICC) expression of CD30, CD15, and PAX5 in Hodgkin's cells (HC) and Reed‐Sternberg cells (RSC) on smears and cell‐blocks (CB) of HL and to compare the performance of each antibody on smears and CB. Methods In 21 FNAC cases of histologically confirmed classical HL, ICC identification of HC and RSC was performed using CD15, CD30, and PAX5 on smears and CB, respectively. Results CD30 was positive in 19/21 cases (90.5%; 11/11 smears and 8/10 CB), CD15 was positive in 14/21 cases (66.7%; 5/11 smears and 9/10 CB), and PAX5 was positive in 13/21 cases (61.9%; 9/11 smears and 4/10CB). Conclusions CD15, CD30, and PAX5 are useful to the FNAC identification of HC and RSC. CD30 is the most sensitive, followed by CD15 and PAX5, which are more effective on CB and smears, respectively.