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Expression of p63 immunostaining in liquid‐based cytology (BD SurePath) of breast fine‐needle aspiration
Author(s) -
Osugi Masumi,
Kinoshita Kosei,
Sugita Atsuro,
Kito Katsumi,
Maeda Toshiharu
Publication year - 2018
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.24041
Subject(s) - medicine , immunostaining , liquid based cytology , fine needle aspiration , fine needle aspiration cytology , cytology , immunohistochemistry , pathology , gynecology , biopsy , cancer , cervical cancer
Background We performed p63 immunostaining to detect myoepithelial cells on BD SurePath liquid‐based cytology (LBC) slides and examined whether this improved the diagnostic accuracy in breast fine‐needle aspiration cytology (FNAC). Methods We examined the diagnostic accuracy using the LBC‐p63 immunostaining slides of 298 lesions obtained from July 2010 to August 2016. Results We defined the cutoff values for malignancy as follows: (1) the percentage of p63+ cluster was <30%, (2) the percentage of p63+ single cells in cell clusters was <3%, (3) the number of p63+ single cells in the background was <20 when the total number of the cell clusters was <100, or the number was <120 when the total number was ≥100. The malignant lesions showed significantly lower values than the benign lesions in the percentage of p63+ clusters, the percentage of p63+ single cells in the clusters, and the number of p63+ single cells in the background ( P < .001). The diagnostic values obtained only by Papanicolaou staining vs the improved values with LBC‐p63 immunostaining slides were as follows. Sensitivity, 88.3% to 99.0%; specificity, 89.1% to 99.0%; positive predictive value, 94.8% to 99.5%; negative predictive value, 77.4% to 97.8%; diagnostic accuracy, 88.6% to 99.0%, respectively. Conclusion The diagnostic accuracy of breast FNAC was significantly improved by adding LBC‐p63 immunostaining.