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The combined application of urinary liquid‐based cytology with fluorescence in situ hybridization and p16/Ki‐67 dual immunostaining is valuable for improving the early diagnosis of upper tract urothelial carcinomas
Author(s) -
Sun Xueqi,
Liu Xubin,
Xia Meng,
Yang Shicong,
Fei Lingyan,
Zhang Mengqi,
Ma Hanyu,
Wang Liantang,
Chen Shangwu,
Yu Li
Publication year - 2017
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.23796
Subject(s) - immunostaining , medicine , fluorescence in situ hybridization , cytology , urinary system , urine , pathology , urine cytology , fish <actinopterygii> , in situ hybridization , upper urinary tract , in situ , urology , immunohistochemistry , biology , cystoscopy , chemistry , biochemistry , gene expression , organic chemistry , fishery , chromosome , gene
Objectives To evaluate the diagnostic values of urine liquid‐based cytology (LBC), fluorescence in situ hybridization (FISH), and p16/Ki‐67 dual immunostaining in the detection of upper tract urothelial carcinomas (UTUCs). Methods Sixty‐one patients with UTUCs were retrospectively analyzed. All patients were diagnosed both by urine cytology and by FISH, and histologically confirmed as UTUCs. Thirty‐two patients had been stained with p16/Ki‐67 dual labeling. Results The sensitivities for low‐grade UTUCs (LGUTUCs) and high‐grade UTUCs (HGUTUCs) were 33.3% and 67.4% by LBC, 60% and 69.6% by FISH, and 12.5% and 66.7% by p16/Ki‐67 dual labeling, respectively. Conclusions Our results indicated that LBC was more suitable to identify HGUTUCs, FISH was highly valuable for predicting LGUTUCs, and p16/Ki‐67 dual labeling was useful for distinguishing HGUTUCs from LGUTUCs. The combined application of these methods may improve the sensitivity or accuracy in the detection or diagnosis of UTUCs.