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Multitarget fluorescence in situ hybridization assay for the detection of lung cancer in bronchial cytology specimens
Author(s) -
Zhai Jing
Publication year - 2015
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.23310
Subject(s) - medicine , cytology , in situ , pathology , fluorescence in situ hybridization , lung cancer , fluorescence , in situ hybridization , lung , biology , biochemistry , optics , gene , chemistry , gene expression , physics , organic chemistry , chromosome
Background Routine bronchial cytology is an important diagnostic tool in suspected lung cancer. The purpose of this study is to evaluate the performance of LAVysion FISH assay over routine cytology for the detection of malignant bronchial cytology specimens. Methods Forty six patients who underwent bronchoscopic examination in a period of 6 months were included. Bronchial brushing and/or washing specimens were accompanied by concurrent endobronchial biopsy and/or FNA, which were used as gold standard for diagnosis. The smears for FISH assay were made from the discarded residual fluid after being processed for routine cytology. Results Twenty seven of the 46 patients were diagnosed of malignancy. For bronchial brushing specimens, the sensitivity of routine cytology and FISH for the detection of malignancy was 18% (3/17) and 35% (6/17) respectively (p = 0.08); the specificity of routine cytology and FISH was the same, 80% (8/10). For bronchial washing specimens, the sensitivity of routine cytology and FISH for the detection of malignancy was 8% (2/24) and 29% (7/24) respectively (p = 0.01); the specificity of routine cytology and FISH was the same, 94% (16/17). Conclusions Our data shows that routine bronchial cytology appears to have a very low sensitivity for the detection of malignancy. FISH assay appears to increase the sensitivity for the detection of malignancy, but does not appear to improve it to a desirable level. A larger prospective study is necessary to further evaluate the role of FISH assay in the detection of malignancy in bronchial cytology specimens. Diagn. Cytopathol. 2015;43:819–824. © 2015 Wiley Periodicals, Inc.