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Mediastinal seminoma: A case report with special emphasis on SALL4 as a new immunocytochemical marker
Author(s) -
Iwamoto Nozomi,
Ishida Mitsuaki,
Yoshida Keiko,
Kagotani Akiko,
Iwai Muneo,
Okabe Hidetoshi
Publication year - 2013
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.22826
Subject(s) - seminoma , medicine , pathology , thymoma , germ cell tumors , immunostaining , mediastinal tumor , cytology , cytopathology , fine needle aspiration , mediastinum , immunohistochemistry , radiology , biopsy , chemotherapy
Cytological diagnosis is a valuable method for detection of mediastinal tumors, and recent reports have shown the usefulness of fine‐needle aspiration cytology for diagnosis of mediastinal tumors, including germ cell tumors. We report a case of mediastinal seminoma diagnosed intraoperatively by cytological examination of cystic fluid containing tumor cells. An anterior mediastinal tumor with cystic component was incidentally found in a 28‐year‐old Japanese male. Cytological examination of the cystic fluid at the time of tumor resection showed single and loose aggregates of large round to polygonal cells with large round nuclei, vesicular chromatin and nucleoli, and mild to moderate amounts of PAS‐positive cytoplasm admixed with mature lymphocytes. A diagnosis of seminoma was made intraoperatively. On subsequent immunostaining, the tumor cells showed nuclear positivity for SALL4, a recently recognized germ cell marker that, in the appropriate setting, can be helpful to distinguish germ cell tumors from other mediastinal neoplasms, such as malignant lymphoma, thymoma, and thymic carcinoma. Diagn. Cytopathol. 2013;41:821–824. © 2012 Wiley Periodicals, Inc.