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Molecular genetic characterization of lymphoma: Application to cytology diagnosis
Author(s) -
Ochs Rachel C.,
Bagg Adam
Publication year - 2012
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.22819
Subject(s) - cytology , medicine , polymerase chain reaction , lymphoma , pathology , fluorescence in situ hybridization , fish <actinopterygii> , cytogenetics , in situ hybridization , genetic testing , comparative genomic hybridization , gene , biology , genetics , genome , gene expression , fishery , chromosome
Mature B‐ and T‐cell lymphomas are increasingly being associated with specific genetic alterations; characterization of these changes can sometimes be crucial to both diagnosis and prognosis. Molecular testing encompasses florescence in situ hybridization (FISH) and polymerase chain reaction (PCR)‐based assays, as well as classical cytogenetics. FISH and PCR‐based assays can be performed on cytology material without the need for advanced planning for testing with fresh tissue. As many lymphoproliferations are amenable to fine needle aspiration diagnosis, it is important for the cytopathologist to be aware of what testing is feasible in these (sometimes limited) specimens. Here, we review pertinent genetic changes in mature B‐ and T‐cell lymphomas with a focus on those that may be encountered more commonly in cytology specimens. Diagn. Cytopathol. 2012;40:542–555. © 2012 Wiley Periodicals, Inc.