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Expression of ki‐67 as proliferation biomarker in imprint smears of endometrial carcinoma
Author(s) -
Kosmas K.,
Stamoulas M.,
Marouga A.,
Kavantzas N.,
Patsouris E.,
Athanassiadou P.
Publication year - 2013
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.21825
Subject(s) - medicine , endometrium , carcinoma , immunostaining , ki 67 , endometrial cancer , pathology , lymph node , clear cell carcinoma , adenocarcinoma , immunohistochemistry , immunocytochemistry , cancer
The aims of this study were to determine the expression of Ki‐67 in type I and type II endometrial adenocarcinomas as well as normal endometrium in imprint smears and to correlate the results with clinicopathologic parameters of primary untreated endometrial cancer patients. During a 29‐month period, 255 patients were evaluated with entometrial imprint cytology. Endometrial samples freshly resected from women who underwent total abdominal hysterectomy were studied. One hundred twenty‐six patients had endometrial carcinoma and 129 cases were diagnosed as normal endometrium. The expression of Ki‐67 was assessed by immunocytochemistry. Positive staining was correlated with increased stage, grade and lymph node metastases. High expression was more frequent in type II than type I endometrial adenocarcinoma and high‐grade endometrial carcinoma had higher proportions of Ki‐67 positive immunostaining compared with low‐grade carcinoma. Proliferative endometrium showed high Ki‐67 expression level, even higher than those of grade 1 and type I. On the other hand, secretory endometrium Ki‐67 positive cells were markedly diminished and even disappeared. Completely negative staining was found to be related to atrophic endometrium. Immunocytochemical findings from Ki‐67 stain, in addition to cytomorphologic features, appeared to be useful for the diagnosis of endometrial carcinoma in endometrial cytology with imprint smears. High Ki‐67 expression correlates with morphologic features of aggressiveness and the expression pattern of Ki‐67 correspond to the expected cyclic/atrophic pattern in normal endometrium. Diagn. Cytopathol. 2013. © 2011 Wiley Periodicals, Inc.