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Expression of Epstein‐Barr virus and granzyme B in cytologic smears of histiocytic necrotizing lymphadenitis
Author(s) -
Kim Jung Yeon,
Shin Eunah,
Kim HyunJung,
Park Kyeongmee
Publication year - 2012
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.21644
Subject(s) - pathology , granzyme b , medicine , cd20 , cd68 , histiocyte , immunohistochemistry , granzyme , in situ hybridization , cd3 , epstein–barr virus , cd8 , virus , immunology , biology , antigen , gene expression , perforin , biochemistry , gene
Abstract Histiocytic necrotizing lymphadenitis (HNL) is a non‐neoplastic disease of the lymph nodes that is self‐limiting in its clinical course. In this study, the expression of Epstein‐Barr virus (EBV), granzyme B, and other phenotypic markers of HNL was investigated in fine needle‐aspirated (FNA) cytologic smears obtained from 38 patients with HNL. The smear were subjected to immunohistochemical staining for granzyme B, CD3, CD4, CD8, CD20, and CD68 in addition to in‐situ hybridization for EBV to determine whether marker expression could be correlated with disease pathogenesis. The mean age of 28 female and 10 male patients was 22.8 years. CD8‐positive cytotoxic T cells were noted in 65.0% of the smears (13/20 cases), whereas CD4 and CD68 were rarely observed. Granzyme B reactivity was seen in lymphocytes, especially in apoptotic areas, and in histiocytes, with positive rates of 25.0% (9/36) and 11.1% (4/36), respectively. Most FNA smears showed immunoreactivity to both CD3 and CD20, with a predominance of CD3‐positive cells. In‐situ hybridization for EBV was positive in 22.9% (8/35) of the cases. The immunohistochemical staining and EBV in‐situ hybridization results obtained in bleached FNA smears were similar to those in histologic sections. Overall, our results implicate that even though EBV positivity and granzyme B immunoreactivity are noted in HNL, they do not appear to have any apoptosis‐associated role. Diagn. Cytopathol. 2012. © 2011 Wiley Periodicals, Inc.