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Significance of cell proliferation markers (Minichromosome maintenance protein 7, topoisomerase IIα and Ki‐67) in cavital fluid cytology: Can we differentiate reactive mesothelial cells from malignant cells?
Author(s) -
Kimura Fumikazu,
Kawamura Jumpei,
Watanabe Jun,
Kamoshida Shingo,
Kawai Kenji,
Okayasu Isao,
Kuwao Sadahito
Publication year - 2010
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.21190
Subject(s) - mesothelial cell , pathology , ki 67 , proliferation marker , immunoperoxidase , biology , immunohistochemistry , microbiology and biotechnology , medicine , monoclonal antibody , antibody , immunology
The aim of this study was to evaluate whether immunocytochemical expressions of proliferation markers, such as minichromosome maintenance protein 7 (MCM 7), topoisomerase IIα (topo IIα), and Ki‐67, in reactive mesothelial cells and malignant cells obtained from cavital fluids could be useful for their differential diagnosis. Samples diagnosed as reactive mesothelial cells (14 cases) or malignant tumors (28 cases) in cavital fluids were examined. Immunocytochemical staining of MCM 7, topo IIα, and Ki‐67 was performed with the universal immunoperoxidase polymer method. In reactive mesothelial cells, MCM 7 was stained in a fine granular pattern and its distribution was uniform in the nuclei. Topo IIα and Ki‐67 were stained in a coarse granular pattern and the distributions were the same as MCM 7. In contrast, in malignant cells, MCM 7 was stained in an irregular and fine granular pattern, and topo IIα and Ki‐67 were stained in a uniform and coarse granular pattern. Labeling indices of MCM 7 (cut‐off value; 30%, sensitivity; 100%, and specificity; 100%), topo IIα (cut‐off value; 15%, sensitivity; 89.3%, and specificity; 92.9%) and Ki‐67 (cut‐off value; 30%, sensitivity; 64.3%, and specificity; 92.9%) of malignant cells were significantly higher than those of reactive mesothelial cells. MCM 7, topo IIα, and Ki‐67 are different types of cell proliferation markers. MCM 7 and topo IIα, in particular, could be reliable tools for differential diagnosis between reactive mesothelial cells and malignant cells. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.