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Are adjunctive markers useful in routine cervical cancer screening? Application of p16 INK4a and HPV‐PCR on ThinPrep samples with histological follow‐up
Author(s) -
Schledermann D.,
Andersen B. T.,
Bisgaard K.,
Dohse M.,
Ejersbo D.,
Hoelund B.,
Horal P.,
Lindh M.,
Ryd W.
Publication year - 2008
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20822
Subject(s) - medicine , cytology , histology , polymerase chain reaction , cervical cancer , human papillomavirus , pathology , gold standard (test) , cancer , biology , biochemistry , gene
The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16 INK4a as a marker for high‐grade cervical lesions, 2) the results of a real‐time polymerase chain reaction detecting high‐risk human papillomavirus, and 3) the interobserver variability of the p16 INK4a interpretation. A total of 232 ThinPrep samples were stained for p16 INK4a , and HPV‐DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow‐up information was collected. The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16 INK4a and 100% for HR‐HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16 INK4a had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow‐up procedures compared to six patients (21%) with the HR‐HPV DNA test. Diagn. Cytopathol. 2008;36:453–459. © 2008 Wiley‐Liss, Inc.