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Accuracy of herpes simplex virus detection in liquid‐based (SurePath) Papanicolaou tests: A comparison with polymerase chain reaction
Author(s) -
Aslan Deniz L.,
Pambuccian Stefan E.,
Prekker Frances L.,
Schacker Timothy W.,
Southern Peter,
Savik Kay,
McKeon Dan,
Gulbahce H. Evin
Publication year - 2008
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20732
Subject(s) - medicine , papanicolaou stain , herpes simplex virus , primer (cosmetics) , polymerase chain reaction , hsl and hsv , cytology , papanicolaou test , liquid based cytology , real time polymerase chain reaction , virology , pathology , virus , biology , cancer , cervical cancer , biochemistry , chemistry , organic chemistry , gene
A review of our institution's Papanicolaou test records over an 11‐yr period showed that liquid‐based Papanicolaou tests (LBPTs) had a significantly higher frequency of diagnoses of Herpes simplex virus (HSV)‐related cellular changes compared to conventional Papanicolaou smears (77/302,841, 0.026% vs. 56/376,173, 0.015%, P = 0.002). To investigate the accuracy of the diagnosis of HSV by LBPT, we performed conventional polymerase chain reaction (PCR) on the residual samples from 258 prospectively collected LBPT and real‐time PCR using a different primer set on a subset of 40 LBPT. Conventional PCR was positive in 22 of 22 cases diagnosed of HSV, 1 of 2 cases diagnosed as suspicious for HSV, and none of 234 LBPT without a cytologic HSV diagnosis. Real‐time PCR was positive in 8 of 8 cases diagnosed as HSV and none of the 32 controls. We conclude that LBPT allows an increased detection of HSV that is highly accurate. Diagn. Cytopathol. 2008;36:94–103. © 2008 Wiley‐Liss, Inc.