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Molecular detection of Chlamydia trachomatis and HPV infections in cervical samples with normal and abnormal cytopathological findings
Author(s) -
de Paula Francisco Danilo Ferreira,
Fernandes Ana Paula,
Carmo Bianca Bianco do,
Vieira Daniela Cristina Duarte,
Dutra Miriam Santos,
Santos Caleb Guedes Miranda dos,
Souza Maria do Carmo M.,
Andrade Túlio César Alvim,
Vago Annamaria Ravara,
Fernandes Paula Ávila
Publication year - 2007
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20629
Subject(s) - ascus (bryozoa) , chlamydia trachomatis , medicine , squamous intraepithelial lesion , hpv infection , pathology , lesion , cytopathology , cervical cancer , chlamydia , cytology , cervical intraepithelial neoplasia , cancer , gynecology , biology , immunology , ascospore , spore , botany
It has been suggested that Chlamydia trachomatis (CT) and human papillomaviruses (HPV) co‐infection could contribute to development of intraepithelial lesions. In this study, HPV and CT‐DNA were investigated in 250 cervicovaginal samples of patients from Minas Gerais, Brazil. The cytological analysis revealed that 70% of samples (175) were negative, 5.2% (13) presented atypical squamous or glandular cells of undetermined significance (ASCUS/AGUS), 12.4% (31) presented low‐grade squamous intraepithelial lesion (LSIL), 10.8% (27) high‐grade squamous intraepithelial lesion (HSIL), and 1.6% (4) invasive carcinoma. HPV‐DNA and HPV/CT co‐infection was observed in 40% (100/250) and in 5.2% (13/250) of samples, respectively. Among the positive cytological samples, HPV‐DNA was detected in 73.3% and CT‐DNA in 9.33% and in 13%, if only the HPV positive samples were considered. The highest co‐infection rate (15.4%) was observed among ASCUS/AGUS samples. Although a significant association was found for HPV infection and the precursor lesions of cervical cancer, it was not possible to establish a significant association between these lesions and CT or HPV/CT co‐infection. Diagn. Cytopathol. 2007;35:198–202. © 2007 Wiley‐Liss, Inc.