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Fine‐needle aspiration cytology combined with flow cytometry immunophenotyping is a rapid and accurate approach for the evaluation of suspicious superficial lymphoid lesions
Author(s) -
Mathiot Claire,
Decaudin Didier,
Klijanienko Jerzy,
Couturier Jérôme,
Salomon Anne,
Dumont Janine,
Vielh Philippe
Publication year - 2006
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20487
Subject(s) - medicine , immunophenotyping , flow cytometry , pathology , fine needle aspiration cytology , cytology , fine needle aspiration , cytopathology , radiology , biopsy , immunology
The authors report a prospective study on 88 samples of superficial lesions (lymph nodes, skin nodules, and breast tumors), performed by fine‐needle aspiration cytology (FNAC) in 74 patients, allowing (i) morphologic analysis combined with immunophenotyping by flow cytometry (FCM) and (ii) a cytogenetic study in 33 cases. Thirty‐nine FNAC (44.3%) were performed at the time of diagnosis. The cytology results were correlated with histopathologic examination in 32 cases. Forty‐nine FNAC (55.7%) were performed in the context of follow‐up of a lymphoma and the results were correlated with those of histopathologic examination in 14 cases. In this study, the concordance between FNAC plus FCM and histopathologic examination was 90% for low‐grade non‐Hodgkin's malignant lymphomas (NHLs) and 83% for high‐grade NHL. The limits of this morphologic and phenotypic approach are (i) partial tumor infiltrations, (ii) Hodgkin lymphoma, and (iii) T‐cell NHL. In conclusion, it may be said that this combined approach is very useful for diagnosis and follow‐up of patients but requires teams experienced in the sampling technique and the morphologic diagnosis of the various types of low‐grade NHL in which supplementary ancillary studies may be performed when morphology and flow cytometry immunophenoyping are not conclusive. Diagn. Cytopathol. 2006;34:472–478. © 2006 Wiley‐Liss, Inc.