Premium
Expression of DNA mismatch repair gene MSH2 in cytological material from lung cancer patients
Author(s) -
Kanellis George,
Chatzistamou Ioulia,
Koutselini Helen,
Politi Ekaterini,
Gouliamos Athanasios,
Vlahos Lambros,
Koutselinis Antonis
Publication year - 2006
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20473
Subject(s) - medicine , msh2 , lung cancer , gene , cancer research , cancer , dna repair , dna , gene expression , dna mismatch repair , pathology , genetics , biology , colorectal cancer
Abstract Mismatch repair genes encode for proteins responsible for the correction of bases incorrectly paired in the DNA. Loss of DNA mismatch repair activity has been associated with various cancers including tumors of the lung. In the present study, we have analyzed by immunocytochemistry the expression of MSH2 DNA repair gene in cytological material obtained by fine needle aspiration from a panel of 42 primary lung cancer patients. Specimens included 13 adenocarcinomas, 11 small cell carcinomas and 18 squamous cell carcinomas. Loss of expression or low expression was detected in 6 out of 13 (46%) adenocarcinomas and in 7 out of 18 (39%) of squamous cell carcinomas, although all 11 small cell carcinomas expressed MSH2. Our results suggest that loss of MSH2 expression is frequent in nonsmall cell carcinomas of the lung ( P < 0.01, χ 2 test). Evaluation of MSH2 expression can be applied for the screening of cytological material from fine needle aspirations from the lung. Diagn. Cytopathol. 2006;34:463–466. © 2006 Wiley‐Liss, Inc.