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Fluorescence in situ hybridization, comparative genomic hybridization, and other molecular biology techniques in the analysis of effusions
Author(s) -
ReisFilho Jorge Sérgio,
de Landér Schmitt Fernando Carlos
Publication year - 2005
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20278
Subject(s) - comparative genomic hybridization , molecular cytogenetics , fluorescence in situ hybridization , cytogenetics , in situ hybridization , pathology , loss of heterozygosity , computational biology , medicine , molecular pathology , biology , genetics , chromosome , gene , gene expression , allele
Over the last 20 yr, the introduction of immunocytochemistry as a diagnostic tool has dramatically revolutionized diagnostic pathology. With the introduction of molecular methods as part of the diagnostic armamentarium, the practicing pathologist is facing the new challenge of grasping novel concepts of the molecular cytogenetics era. Herein, we review the diagnostic contribution of ancillary molecular techniques, including fluorescent and chromogenic in situ hybridization, telomerase assays, loss of heterozygosity, comparative genomic hybridization (CGH), and microarray‐based CGH, for the practicing cytopathologists and discuss how these techniques will help pathologists in decision‐making. Diagn. Cytopathol. 2005;33:294–299. © 2005 Wiley‐Liss, Inc.