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MIB‐1 and PCNA immunostaining as a diagnostic adjunct to cervical Pap smear
Author(s) -
Goel M.M.,
Mehrotra Anju,
Singh Uma,
Gupta H.P.,
Misra J.S.
Publication year - 2005
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20218
Subject(s) - proliferating cell nuclear antigen , medicine , pathology , immunostaining , cervical intraepithelial neoplasia , papanicolaou stain , immunohistochemistry , staining , cervical cancer , cancer
The present study was done to determine the role of MIB‐1 (Molecular Immunology Borstel) and proliferating cell nuclear antigen (PCNA) proliferative index as a diagnostic adjunct to cervical Papanicolaou (Pap) smear for the identification of ascending grades of cervical intraepithelial neoplasia (CIN) developing into cancer in the human uterine cervix. A total of 49 adequate Pap smears with consensus diagnosis were destained for immunocytochemical staining (MIB‐1 and PC10). Staining was done by streptavidin‐biotin method after antigen retrieval. MIB‐1 and PC10 labeling index (LI) were calculated in each case and divided into three groups, i.e., <10%, 10–20%, and >20%, respectively. Statistical analysis was done by using the SPSS 10.0 package. The comparisons were made using analysis of variance (ANOVA) and independent sample t ‐test. Bivariate and Pearson's correlation coefficient were used to obtain correlations between different groups. Out of 49 cases, 40 cases (81.6%) showed positive immunostaining with MIB‐1 and PCNA. Proliferative LI of MIB‐1 and PCNA increased with the ascending grades of CIN lesions to carcinoma. The highest proliferative index (mean ± SD) for PCNA and MIB‐1 were observed for the carcinoma group (PCNA LI, 39.200 ± 1.6865; MIB‐1LI, 35.300 ± 1.8886). A significant positive correlation between ascending grades of squamous intraepithelial lesion (SIL) and labeling indices of markers ( r = 0.87 for MIB‐1 and r = 0.88 for PCNA) suggests that MIB‐1/PCNA proliferative markers can be used as an adjunct to cytomorphological interpretation of conventional cervical Pap smear. Diagn. Cytopathol. 2005;33:15–19. © 2005 Wiley‐Liss, Inc.