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Evaluation of the CD34 and CD10 immunostains using a two‐color staining protocol in liver fine‐needle aspiration biopsies
Author(s) -
Zimmerman Robert L.,
Logani Sanjay,
Baloch Zubair
Publication year - 2005
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20190
Subject(s) - medicine , fine needle aspiration , pathology , immunostaining , staining , cd34 , liver parenchyma , biopsy , immunohistochemistry , stem cell , biology , genetics
Fine‐needle aspiration (FNA) biopsies of liver masses may need immunohistochemistry to resolve difficult cases. We examined liver FNAs with CD10 and CD34 using a two‐color staining protocol. Fifty‐one cases of hepatocellular carcinoma (HCC) and 23 cases of liver metastases were stained first with CD10 using diaminobenzidine as a chromogen and then with CD34 using Fast Red. All cases were reviewed in a blinded fashion by two of the authors (R.I.Z. and Z.B.). Diagnoses were unblinded and staining patterns evaluated. Forty‐eight of 51 cases of HCC stained for CD34 in a peripheral pattern and no cases of metastases stained for CD34 Seven of 23 cases of metastatic disease stained for CD10 in a diffuse cytoplasmic distribution and 29 of 51 HCCs stained in a canalicular pattern. Overall, the sensitivity of the CD10/CD34 combination was 98% and the specificity was 100% for HCC when appropriate staining patterns were observed. Two‐color immunostaining with CD10 and CD34 may prove useful in distinguishing HCC from metastases. Diagn. Cytopathol. 2005;32:88–91. © 2005 Wiley‐Liss, Inc.