Premium
Detection of high‐risk human papillomavirus (HPV) DNA by Hybrid Capture II in women referred due to atypical glandular cells in the primary screening
Author(s) -
Oliveira Eliane R.Z.M.,
Derchain Sophie F.M.,
RabeloSantos Silvia H.,
Westin Maria Cristina A.,
Zeferino Luiz Carlos,
Campos Elisabete A.,
Syrjanen Kari J.
Publication year - 2004
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.20049
Subject(s) - medicine , hybrid capture , human papillomavirus , gynecology , dna testing , cervical cancer , cervical intraepithelial neoplasia , cancer , biology , genetics
We assessed the detection of high‐risk human papillomavirus DNA (HPV‐DNA) in women examined by a second Pap smear due to atypical glandular cells (AGC) detected in their screening Pap smear. In 91 women included in the study, a second Pap smear was taken and HPV‐DNA test was performed using Hybrid Capture II (HC II). The second Pap smear showed no abnormalities in 28 (31%) cases, ACG in 17 (19%) cases, and high‐grade squamous intraepithelial lesions (HSIL) or worse in 24 (26%). HC II test was positive in 36% of the cases. Considering the second Pap smear diagnosis, HPV‐DNA was detected in 87% of the women with HSIL, 100% of women with in situ adenocarcinoma, and only in 11% of the women with no abnormalities. The use of the second Pap smear combined with HPV‐DNA may improve the management of women with AGC in the primary screening. Diagn. Cytopathol. 2004;31:19–22. © 2004 Wiley‐Liss, Inc.