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Cytoplasmic vacuolation, intracytoplasmic lumina, and DPAS staining in ductal carcinoma of the breast
Author(s) -
Nijhawan Raje,
Rajwanshi Arvind,
Gautam Upasana,
Gupta Subhash K.
Publication year - 2003
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.10272
Subject(s) - medicine , pathology , staining , carcinoma , cytoplasm , breast carcinoma , breast cancer , cancer , biology , microbiology and biotechnology
Abstract A retrospective study was carried out on 30 cases of histologically proven invasive ductal carcinoma of the breast with a prior fine‐needle aspiration (FNA) cytology. On evaluating the May‐Grünwald‐Giemsa (MGG) FNA smears, cytoplasmic vacuolation was observed in 70% cases. Positivity with periodic acid Schiff‐positive, diastase‐resistant (DPAS) staining was observed in 90% of cases. The χ 2 value on a McNemar test was 4.16. Thus, DPAS staining was significantly superior to MGG staining for picking up cytoplasmic vacuoles ( P < 0.05). In 56.67% cases, DPAS staining showed an improvement in score as compared to MGG smears. This was highly significant ( P < 0.001) on Wilcoxon matched‐pairs signed‐ranks test. Applying the strict criteria of thick‐walled cytoplasmic vacuoles with a central darkly stained dot, none of our cases revealed true intracytoplasmic lumina. Larger studies are required to establish a role for DPAS staining in separating borderline, in situ, and invasive breast lesions, and to see if such positvity can be incorporated into the grading systems for breast carcinoma. Diagn. Cytopathol. 2003;28:291–294. © 2003 Wiley‐Liss, Inc.