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Ultrafast Papanicolaou stain and cell‐transfer technique enhance cytologic diagnosis of Hodgkin lymphoma
Author(s) -
Zu Youli,
Gangi Maryann D.,
Yang Grace C.H.
Publication year - 2002
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.10180
Subject(s) - medicine , pathology , papanicolaou stain , staining , cd15 , lymphoma , cytology , immunophenotyping , fine needle aspiration , stain , flow cytometry , biopsy , immunology , stem cell , cancer , cd34 , biology , cervical cancer , genetics
Diagnosis of Hodgkin lymphoma (HL) by fine‐needle aspiration (FNA) is often hampered by aspirated blood that camouflage scattered Hodgkin cells and Reed‐Sternberg (HRS) cells, and the absence of HRS cells in the smears submitted for immunophenotyping. The objective of this study was to develop a simple protocol to overcome these problems. The visibility of HRS cells in Diff‐Quik, traditional, and Ultrafast Papanicolaou (UFP) stains in FNA smears were compared in 73 cases of HL. HRS cells were found to be most visible in UFP because of the hemolysis of aspirated blood and the highlighting of HRS cells by the red‐staining nucleoli. UFP‐stained smears containing HRS cells were subsequently immunophenotyped via the cell‐transfer technique. UFP staining was found to have no deleterious effect on the immunoreactivity of cellular CD15 and CD30 antigens of HRS cells. This simple protocol enhances the cytologic diagnosis of HL, feasible even with a single smear. Diagn. Cytopathol. 2002;27:308–311. © 2002 Wiley‐Liss, Inc.