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Immunolocalization of glucose transporter 1 and 3 in the placenta: Application to cytodiagnosis of Papanicolaou smear
Author(s) -
Sato Misako,
Nakamura Yasushi,
Sogawa Tukumi,
Yang Qifeng,
Taniguchi Takeshi,
Taniguchi Emiko,
Kagiya Tomoko,
Nakamura Misa,
Mori Ichiro,
Kakudo Kennichi
Publication year - 2002
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.10124
Subject(s) - glut3 , glut1 , glucose transporter , cytokeratin , immunostaining , medicine , placenta , syncytiotrophoblasts , pathology , immunohistochemistry , biology , endocrinology , pregnancy , fetus , insulin , genetics
A positive immunostaining for glucose transporter 1 (GLUT1) was exclusively localized in microvilli on the free surface of syncytiotrophoblasts in the placenta. An enhanced immunoreaction for glucose transporter 3 (GLUT3) was elicited in the cell membrane of intermediate trophoblasts and cytotrophoblasts. Neither GLUT1 nor GLUT3 was positive in decidual cells and epithelial components from cervical dysplasia and carcinoma in situ. Cervicovaginal smears from six pregnant women containing atypical cells of unknown origin were subjected to immunocytochemical testing with antibodies against GLUT1 and GLUT3. Atypical cells in smears from two pregnant women were found to be positive for GLUT3 while no specific immunoreaction for GLUT1 was elicited, indicating their origin from either intermediate trophoblasts or cytotrophoblasts. Through the use of antibodies against vimentin and cytokeratin 17, GLUT3‐negative atypical cells were further sorted into decidual cells and epithelial components from cervical dysplasia, respectively. Diagn. Cytopathol. 2002;26:373–379. © 2002 Wiley‐Liss, Inc.