Premium
Quantitative FISH analysis on interphase nuclei may improve diagnosis of DNA diploid breast cancers
Author(s) -
Truong Khuong,
Vielh Philippe,
Guilly MarieNöelle,
Klijanienko Jerzy,
SastreGarau Xavier,
Soussaline Françoise,
Dutrillaux Bernard,
Malfoy Bernard
Publication year - 2002
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/dc.10057
Subject(s) - ploidy , interphase , malignancy , flow cytometry , chromosome , fluorescence in situ hybridization , pathology , aneuploidy , dna , fish <actinopterygii> , medicine , biology , microbiology and biotechnology , cancer research , genetics , gene , fishery
The detection of DNA aneuploid cells using flow cytometry is an indication for the presence of tumor cells, but when DNA diploid cells are found in 25–33% of the cases, the diagnostic and prognostic significance of DNA ploidy is more limited. We analyzed interphase nuclei after in situ hybridization and using image cytometry on 50 breast tumors with diploid DNA content to investigate whether early chromosome rearrangements were detectable and if their occurrence was clinically significant. Imbalances between the two arms of chromosome 1 were found in 55% of the cases and values ranged from 1.5–3.0. Comparison with histological data showed that Grade I tumors mainly have imbalances (67%) and that Grade III tumors were mainly without the imbalance (67%), whereas Grade II tumors were intermediate (50% imbalance). These data suggest that the diagnosis of DNA diploid cases may be improved by using interphase FISH. In addition, the data also indicates that early breast tumors may have different genetic origins, which is important in the comprehension of tumor malignancy in early stages, especially for preinvasive lesions. Diagn. Cytopathol. 2002;26:213–216. © 2002 Wiley‐Liss, Inc.