Open AccessThe global Alzheimer's Association round robin study on plasma amyloid β methods
Author(s) -
Pannee Josef,
Shaw Leslie M.,
Korecka Magdalena,
Waligorska Teresa,
Teunissen Charlotte E.,
Stoops Erik,
Vanderstichele Hugo M. J.,
Mauroo Kimberley,
Verberk Inge M. W.,
Keshavan Ashvini,
Pesini Pedro,
Sarasa Leticia,
PascualLucas Maria,
Fandos Noelia,
Allué JoséAntonio,
Portelius Erik,
Andreasson Ulf,
Yoda Ritsuko,
Nakamura Akinori,
Kaneko Naoki,
Yang ShiehYueh,
Liu HueiChun,
Palme Stefan,
Bittner Tobias,
Mawuenyega Kwasi G.,
Ovod Vitaliy,
Bollinger James,
Bateman Randall J.,
Li Yan,
Dage Jeffrey L.,
Stomrud Erik,
Hansson Oskar,
Schott Jonathan M.,
Blennow Kaj,
Zetterberg Henrik
Publication year - 2021
Publication title -
alzheimer's and dementia: diagnosis, assessment and disease monitoring
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.497
H-Index - 37
ISSN - 2352-8729
DOI - 10.1002/dad2.12242
Subject(s) - cerebrospinal fluid , alzheimer's disease , measure (data warehouse) , blood plasma , amyloid beta , amyloid (mycology) , antibody , chemistry , medicine , pathology , immunology , psychology , disease , computer science , database
Blood‐based assays to measure brain amyloid beta (Aβ) deposition are an attractive alternative to the cerebrospinal fluid (CSF)–based assays currently used in clinical settings. In this study, we examined different blood‐based assays to measure Aβ and how they compare among centers and assays. Methods Aliquots from 81 plasma samples were distributed to 10 participating centers. Seven immunological assays and four mass‐spectrometric methods were used to measure plasma Aβ concentrations. Results Correlations were weak for Aβ42 while Aβ40 correlations were stronger. The ratio Aβ42/Aβ40 did not improve the correlations and showed weak correlations. Discussion The poor correlations for Aβ42 in plasma might have several potential explanations, such as the high levels of plasma proteins (compared to CSF), sensitivity to pre‐analytical sample handling and specificity, and cross‐reactivity of different antibodies. Different methods might also measure different pools of plasma Aβ42. We, however, hypothesize that greater correlations might be seen in future studies because many of the methods have been refined during completion of this study.