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Determination of CD43 and CD200 surface expression improves accuracy of B‐cell lymphoma immunophenotyping
Author(s) -
Hoffmann Joerg,
Rother Marissa,
Kaiser Ulrich,
Thrun Michael C.,
Wilhelm Christian,
Gruen Andrea,
Niebergall Ute,
Meissauer Ute,
Neubauer Andreas,
Brendel Cornelia
Publication year - 2020
Publication title -
cytometry part b: clinical cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 61
eISSN - 1552-4957
pISSN - 1552-4949
DOI - 10.1002/cyto.b.21936
Subject(s) - cd43 , immunophenotyping , chronic lymphocytic leukemia , lymphoma , medicine , b cell , antigen , cd20 , gastroenterology , pathology , leukemia , oncology , immunology , antibody
Background The Matutes score (MS) was proposed to differentiate chronic lymphocytic leukemia (CLL) from other B‐cell non‐Hodgkin lymphomas (B‐NHLs). However, ambiguous immunophenotypes are common and remain a diagnostic challenge. Therefore, we evaluated the diagnostic benefit of measuring CD200 and CD43 expression together with the standard MS antigens. Methods 138 lymphoma patient samples and a validation cohort of 138 additive samples were classified according to the standard MS and further assigned with one or two additional points, for high CD200 and/or CD43 expression levels. The “ classical ” MS and the “ Matutes score‐extended ” (MS‐e) were categorized as high (4‐5/6‐7), intermediate (2‐3/4‐5), and low (0‐1/0‐3). Samples were reclassified into the MS‐e with focus on ambiguous cases with an intermediate “ classical ” MS. Results A total of 35 of 138 (25.4%) patient samples were assigned to the intermediate MS group and confirmed by histopathological reports as CLL (14/40.0%) and B‐NHLs other than CLL (21/60%). MS‐e analysis identified 13 of 14 (92.9%) of CLL cases (MS‐e 4–5) and 18/21 (85.7%) non‐CLL cases (MS‐e ≤ 3) correctly. Overall, the sensitivity of the CLL diagnosis was significantly increased by application of MS‐e compared to the “ classical ” MS (98.8% vs. 82.7%; p = 0.0009), while specificity of both methods was almost equal (94.7% vs. 98.3%; p = 0.4795). Of note, sole measurement of CD43 and CD200 on B‐cells sufficiently differentiated CLL from non‐CLL with a test accuracy superior to the “ classical ” MS (F1 score 96.2 vs. 93.6). Conclusion CD200 and CD43 have a high informative value in diagnostic immunophenotyping and facilitate the separation of CLL from other B‐NHLs particularly in ambiguous cases.